Targeting and fusion proteins during mammalian spermiogenesis
نویسندگان
چکیده
منابع مشابه
Membrane flow during nematode spermiogenesis
Two distinct types of surface membrane rearrangement occur during the differentiation of Caenorhabditis elegans spermatids into amoeboid spermatozoa. The first, detected by the behavior of latex beads attached to the surface, is a nondirected, intermittent movement of discrete portions of the membrane. This movement starts when spermatids are stimulated to differentiate and stops when a pseudop...
متن کاملTransient production of scFv-Fc fusion proteins in mammalian cells
Single chain Fv (scFv) antibody fragments fused to the human immunoglobulin G (IgG) Fc moiety obtain IgG-like properties but nevertheless they are still encoded by a single gene. Since transient production of scFv-Fc proteins in mammalian can easily achieve milligram amounts, this antibody format is particularly suitable for many research applications. Here, we describe two protocols for the tr...
متن کاملMitochondria: transportation, distribution and function during spermiogenesis
Spermiogenesis is a dynamic process which includes organelle reorganization and new structure formation. The morphology and distribution of the mitochondria in germ cells change to accommodate the cellular requirement. Multiple molecular motors and related proteins participate in carrying and anchoring mitochondria to the midpiece during spermiogenesis and this process is regulated precisely. E...
متن کاملRAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis
According to recent estimates, 2%-15% of couples are sterile, and approximately half of the infertility cases are attributed to male reproductive factors. However, the reasons remain undefined in approximately 25% of male infertility cases, and most infertility cases exhibit spermatogenic defects. Numerous genes involved in spermatogenesis still remain unknown. We previously identified Male Ger...
متن کاملTargeting scFv-Fc-scTRAIL fusion proteins to tumor cells
Fusion proteins combining hexavalent TRAIL with antibody fragments allow for a targeted delivery and efficient apoptosis induction in tumor cells. Here, we analyzed scFv-Fc-scTRAIL molecules directed against EGFR, HER2, HER3, and EpCAM as well as an untargeted Fc-scTRAIL fusion protein for their potentials to induce cell death both in vitro and in a xenograft tumor model in vivo. The scFv-Fc-sc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biological Research
سال: 2001
ISSN: 0716-9760
DOI: 10.4067/s0716-97602001000200021